Content determination of Rutin in Hibiscus mutabilis L by HLPC
- VernacularTitle:HLPC法测定木芙蓉提取物中芦丁含量
- Author:
Qinhai SHEN
- Publication Type:Journal Article
- Keywords:
RT-HLPC;
H ibiscus mutabilis L;
Rutin
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective; To establish a method for content determinate of Rutin in H ibiscus mutabilis L by RT- HLPC. Methods; The chromatographic conditions were as follows; Column; Ubondpak C18; the column temperature was 25℃and the detection wavelength was 370nm;Mobil phase consisted of 0.02mol/L KH2PO4 solution cotaining 1.0% glacial acetic acid -methanol (73:27, v:v). The flow rate was 1.0ml/min. SPD -2ASUV detector was used. The standard sample was used as control. Results; Good linearity was obtained for Rutin and other components in the range of (0.5-5) ?g/20?l. Peak area was favorable liner between the concentration of Rutin in range . The regression equation was Y =0.0001X +0. 1208,correlation coefficient r = 99.8% ,The recovery rate was 99. 88% , RSD was 1. 54% (n = 5 ). Conclusion; The method is convenient, precise, resulting in high separate efficacy, and suited for the content determination of Rutin in H ibiscus mutabilis L.
