Content Determination of Trigonelline in Radix Mirabilis by RP-HPLC
- VernacularTitle:HPLC法测定紫茉莉根中葫芦巴碱的含量
- Author:
Fenyun SONG
;
Dezhi ZHANG
;
Yunxiang ZHONG
;
Wei WANG
;
Shaohu ZENG
- Publication Type:Journal Article
- Keywords:
Radix Mirabilis;
Trigonelline;
RP-HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of trigonelline in Radix Mirabilis.Methods A RP -HPLC method was established.The chromatographic c olumn was Inertsil NH 2 Columns(250?4.6mm,5?m.).The mobile phase was acetonitrile -water(80∶20).The flow rate was 0.8mL?min -1 and the detection wavelength was at 265nm,and the column temperature was 30℃.Results The calibration curve was linear in t he range of 0.1872～0.9360?g .The aver-age recovery for trigonelline was 99.6%and RSD was 1.76%(n=6).Conclusion The method is simple and effec-tive and can be used to determine the c ontent of trigonelline in Radix Mirabilis.
