Determination of Tetrahydropalmatione in FuLe Granules by HPLC
- VernacularTitle:高效液相色谱法测定妇乐颗粒中延胡索乙素的含量
- Author:
Jianying ZHAO
;
Weiyuan BAO
- Publication Type:Journal Article
- Keywords:
FuLe granules;
Tetrahydropalmatione;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of tetrah yd ropalmatione in FuLe granules. Methods A HPLC method was used with DiamonsilTM C 18(250? 4.6 mn, 5 ? m) column and a mobile phase of methanol- 0.1 % phospho ric acid solution (adjusted the pH to 6.0 with triethylamine)(60 ∶ 40). Flow ra te was 1.0 mL/min, column tem perature 35 ℃ , the detection wavelength at 280nm and sample loop volume bei ng 20 ? L. Results The calibration curve was linear within the range of 0.102 ～ 1.632 ? g of tetrahydropalmatione, the average recovery was 98.78 % (RSD=2 .75 % ).Conclusion The method is simple and accurate.It can be used for the q uality control of Fule granules.
