Effect of gene expression of bcl-2 and bcl-xL in rabbit myocardium by Ginko biloba extract GBE50
- VernacularTitle:银杏叶提取物GBE50对兔心肌组织bcl-2、bcl-xL基因表达的影响
- Author:
Feng ZHANG
;
Runfen CHEN
;
Huili LI
;
Dingjiu HUANG
- Publication Type:Journal Article
- Keywords:
Ginko biloba extract;
bcl-2 bcl-xL;
gene expression
- From:
Chinese Traditional Patent Medicine
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate affection of the novel Ginko biloba extract GBE50 on bcl-2、bcl-xL gene expression in rabbit myocardium. METHODS: Adult male New Zealand rabbits were randomly divided into 2 groups. GBE50 group were orally administrated with 100 mg/kg GBE50 daily. Control group were only administrated vehicle 2% CMC-Na. After being raised for 4 weeks, the animals were killed and the ventricular tissue were excised. We performed reverse transcriptase polymerase (RT-PCR) to measure gene expression of bcl-2、bcl-xL. Semiquantitative analysis method based on gel electrophoresis image was adopted to compare gene expression among groups. RESULTS : The gene expression of bcl-2、bcl-xL significantly increased in GBE50 group compared with control group. CONCLUSION: The novel Ginko biloba extract GBE50 can promote expression of the antiapoptotic gene bcl-2、bcl-xL in rabbit myocardium.
