IMMUNOGOLD LOCALIZATION OF GFAP AND MDR1 IN SURGICALLY RESECTED BRAIN TISSUES FROM CLINICAL INTRACTABLE EPILEPSY PATIENTS BY TRANSMISSION ELECTRON MICROSCOPY
- VernacularTitle:难治性癫痫患者脑组织胶质原纤维酸性蛋白和多药耐药基因蛋白的免疫电镜观察
- Author:
Chengyun DING
;
Qunyuan XU
;
Guoming LUAN
- Publication Type:Journal Article
- Keywords:
Intractable epilepsy;
MDR1;
GFAP;
Immunoelectron microscopy;
Man
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the ultrastructural localization of MDR1 and GFAP in the surgically resected brain tissues from intractable epilepsy patients. Methods Expression of MDR1 and GFAP in brain tissues was examined by using PAG immunolabeling technique for electron microscopy. Results The MDR1 and GFAP labeled by gold particles were only detected at some reactive astrocytes. The positive gold particles were mainly located in the astrocytic cytoplasm and their membrane, but not in the nucleus.Conclusion The expression of MDR1 and GFAP in the brain of patients with clinically intractable epilepsy were mainly located at the cytoplasm and membrane of certain reactive astrocytics.;
