Analyze the abnormal expression of Blys and Blys mRNA on peripheral blood mononuclear cells from patients with SLE and its clinical significance
- VernacularTitle:系统性红斑狼疮患者外周血单个核细胞B细胞刺激因子的异常表达及其临床意义
- Author:
Yujin YE
;
Peida YIN
;
Xueqing YU
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
B lymphocyte stimulator;
Peripheral blood mononuclear cells
- From:
Chinese Journal of Rheumatology
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the expression of membrane-bound B lymphocyte stimulator (Blys) and Blys mRNA in peripheral blood mononuclear cells (PBMCs) from patients with systemic lupus erythematosus (SLE), and investigate the relationship between Blys mRNA expression and SLE activity or lupus nephritis. Methods Measure the expression of Blys mRNA with reverse transcription-PCR (RT-PCR) in PBMCs from patients with SLE, who were separated into active group (n=26) and inactive group (n=20) according to the SLE disease activity index (SLEDAI) and 20 healthy volunteers; analyze the relationship among Blys mRNA and several clinical items; measure the expression of membrane-bound Blys with flow cytometry (FACs) in 20 patients with SLE and 16 healthy controls. Results The expression of Blys mRNA in PBMCs from patients with SLE was significantly elevated compared to healthy controls (P
