Determination of Saponins Content in Compound Danshen Prescription by HPLC
- VernacularTitle:HPLC测定复方丹参方中三七皂苷类成分的含量
- Author:
Guixiang PAN
;
Xiumei GAO
;
Boli ZHANG
- Publication Type:Journal Article
- Keywords:
@ Compound Danshen Prescription/chemistry;
Notoginsenoside R1/analysis;
Ginsenoside Rg1/analysis;
Ginsenoside Re/analysis;
Ginsenoside Rb1/analysis;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for the determination of four saponins of panax notoginseng in Compound Dan-shen Prescription (CDP) by HPLC. Methods The conditions for the experiment were: determination of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Re with acetonitrile - 0. 05 % phosphoric acid (19 : 81) as mobile phase and the detection wave at 203nm, determination of ginsenoside Rb1 with acetonitrile - water (33 : 67) as mobile phase and the detection wave at 203nm. Results A good linearity for notoginsenoside R1 was in the range of 0. 24 ~ 3. 6?g ( r = 0. 9997), 1. 2 ~ 18?g (r = 0. 9994) for ginsenoside Rg1, 0. 08 ~ 1. 2?g(r = 0. 9997) for ginsenoside Re and 0. 25-3. 72?g (r = 0. 9998) for ginsenoside Rb1. The stability of the four saponins is well kept at 4℃ for four months. Conclusion This method is simple, rapid, accurate and with good reproducibility and can be used for the quality control of CDP.
