In situ HYBRIZATION STUDY ON GAP-43mRNA EXPRESSION DURING NERVE INJURE
- VernacularTitle:神经损伤与再生中神经元GAP-43mRNA表达的原位杂交研究
- Author:
Guangjiu LIU
;
Zhenqiang LI
;
Yuqin YIN
;
Lin SONG
;
Jiansen SUN
- Publication Type:Journal Article
- Keywords:
Nerve injury;
GAP\|43;
In situ hybridization;
Rat
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study GAP\|43mRNA expression during nerve injure and regeneration. Methods Rat sciatic nerve was crushed then, in situ hybridization technique was used to explore GAP\|43mRNA expressions in spinal cord and dorsal root ganglion (DRG). Results The neurons of spinal cord and DRG were detected to have GAP\|43 hybridization sinal by 2 days after sciatic nerve lesion. At later times(4,7 and 14 days postsurgery) the anterior horn motor neurons and DRG cells showed an increase in the number of GAP\|43mRNA positive neurons, followed by a significant rise in their content of GAP\|43mRNA. The number of GAP\|43mRNA positive neurons was decreased by 30 days postinjure, and was nearly nomal 60 days postinjure.Conclusion GAP\|43mRNA expression was increased during peripheral nerve injure and regeneration. The study showed that GAP\|43 may play a key role in nerve regeneration. [
