Correlation of HR-HPV infection and p16 gene expression in cervical cancer
10.3969/j.issn.1673-4130.2014.11.001
- VernacularTitle:p 16基因表达及高危 HPV 感染在宫颈癌中相关性的研究
- Author:
Wenbing LI
;
Haijun LUO
;
Yue LIU
;
Peng WANG
;
Jicai ZHANG
- Publication Type:Journal Article
- Keywords:
cervical cancer;
p16 gene;
human papilloma virus
- From:
International Journal of Laboratory Medicine
2014;(11):1377-1378,1381
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of p16 and discuss the significance in genesis and development of cervical cancer. Methods FQ-PCR method was used to detect the expression levels of p16 mRNA in cervical cancer(CC),cervical intraepithelial neoplasia(CIN),and normal tissues.And in situ hybridization was used to detect HR-HPV in these tissues.Results The expres-sion levels of p16 were 0.79±0.34,0.70±0.36,0.26±0.21 in CC,CIN and normal cervical tissues,respectively.The difference was significant (P <0.05).The expression level of p16 was significantly associated with FIGO stage (P <0.05),but it showed no significant relationship with age,tumor size,cell differential degree and lymphoid node metastasis (P >0.05).The expression level of p16 in HR-HPV positive cases was significantly higher than that in HR-HPV negative cases (P <0.05).Conclusion p16 shows high expression level in cervical cancer,which is closely related to the infection of HR-HPV infection.It suggests that the high ex-pression of p16 play an important role in genesis and development of cervical cancer.
