CYTOCHEMICAL CHANGES OF HEPATOCYTES IN MICE  EXPOSED TO HYPOXIA

VernacularTitle:低氧下小鼠肝细胞的细胞化学变化
Author: Longmei WA ; Feng GAO ; Xiaobei ZENG
Publication Type:Journal Article
Keywords: Hypoxia; Hepatocyte; Cytochemistry
From: Acta Anatomica Sinica 1955;0(03):-
CountryChina
Language:Chinese
Abstract: Objective To study the cytochemical changes of hepatocytes in mice exposed to hypoxia. Methods The mice were divided into two groups(n=45, all males, hypoxia group=36, control group=9). The mice of the hypoxia group lived in hypoxia (O 2:10%). Using imaging analytical instrument LEICA-500IW, We observed cytochemical changes of the hepatocytes. The PAS, LDH, cytochrome oxidase, Mg 2+ dependent ATPase activity of hepatocytes was assayed. Results Compared with the controls, the LDH activity of hepatocytes increased dramatically, the longer the hypoxic time, the higher the LDH activity of the hepatocytes; the cytochrome oxidase activity of hepatocytes decreased sharply, the longer the hypoxic time, the lower the cytochrome oxidase activity of the hepotocytes.Conclusion Hypoxia can injury the hepatocytes of the mice and result in sharp changes of the hepatic cytochemistry.;