Inhibitory effects of aspirin and indomethacin on the growth of H.pylori in vitro
- VernacularTitle:阿司匹林和吲哚美辛对幽门螺杆菌生长的抑制作用
- Author:
Weihong WANG
;
Fulian HU
;
Zhenyu WANG
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
Aspirin;
Indomethacin
- From:
Chinese Journal of Digestion
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible effects of aspirin and indomethacin on the growth of Helicobacter pylori(H.pylori) and the effects of aspirin on the susceptibility of H.pylori to some antimicrobials. Methods Kinetic studies of H.pylori were performed by inoculating strains in Brucella broth with different concentrations of aspirin and indomethacin. Growth of bacteria in the broth was assessed spectrophotometrically and by viable colony count after incubation for 24 and 48 hours. Bacteria morphology was studied by Gram stain under light microscopy. MICs of aspirin and indomethacin were determined by standard agar dilution method. MICs of amoxicillin, clarithromycin and metronidazole were measured in the presence and in the absence of aspirin by E test method. Results Kinetic studies revealed that aspirin and indomethacin inhibited the growth of H.pylori in a dose dependent manner and the bactericidal activities of these agents were due to cell lysis. Aspirin at 400 ?g/ml produced about 2 logs decrease in CFU per milliliter at 48 hours. The assemble inhibition effects were obtained when 100 ?g/ml indomethacin tested. The MIC 90 of H.pylori for aspirin and indomethacin were 512 ?g/ml and 128 ?g/ml, respectively. Increased susceptibility to amoxicillin, clarithromycin and metronidazole of H. pylori were induced by aspirin. Conclusions Aspirin and indomethacin could significantly inhibit the growth of H.pylori when incubated in Brucella broth in vitro. A subinhibitory concentration of aspirin enhanced the susceptibility of H. pylori to antimicrobial agents.
