Expression of the extracellular domain of human CD7 in Escherichia coli
10.3969/j.issn.1000-8861.2001.02.008
- VernacularTitle:人CD7胞外区基因在大肠杆菌中的表达
- Author:
Yanchun ZHOU
;
Xihua ZHU
;
Yunhui HUANG
- Publication Type:Journal Article
- From:
Immunological Journal
2001;(2):105-106,109
- CountryChina
- Language:Chinese
-
Abstract:
Objective To get the antigen of CD7.Methods The extracellular domain of human CD7 was cloned from a plasmid containing the full length of human CD7 cDNA and expressed in pinpoint-xa3 prokaryotic system. Results Analysis with SDS-PAGE and Western-blotting showed that the expressed protein could bind to the anti-CD7 mAb specifically and is about 30 000 u in molecular weight. Conclusion These results paved the way for preparing anti-CD7 engineering antibody.
