Effects and mechanism of ginsenoside-Rg1 on SK-N-SH cell treated with chronic morphine and naloxone-precipitated withdrawal
- VernacularTitle:人参皂苷Rgl对慢性吗啡作用及纳洛酮催促戒断SK-N-SH细胞的影响及机制研究
- Author:
Yuxian YAN
;
Yueying SONG
;
Xiaoping WANG
;
Haisheng CHEN
;
Chunxiao WEN
- Publication Type:Journal Article
- Keywords:
morphine;
naloxone;
ginsenoside-Rg1;
SK-N-SH cell;
[Ca2 + ]i;
CaMKⅡ?
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects and mechanism of ginsenoside-Rg1 on SK-N-SH cells treated with chronic morphine and naloxone-precipitated withdrawal. Methods Cells were pretreated with ginsenoside-Rg1 1,2,4,8,16,32 ?mol?L-1 for 24 h,then incubated for 24 h with morphine ( 100 ?mol?L-1 ) . MTT colorimetr was used to study the effects of ginsenoside-Rg1 on the multiplication of the cells treated with chro-nic morphine. After stimulated by the same concentra-tion of morphine,cells were added with different concentrations of Rg1 1,2,4 ?mol?L -1 for 24 h before stimulated with 10 ?mol?L -1 NAL. Fuorospectrophotometry RT-PCR and Western blot techniques were used to detect the effects of ginsenoside-Rg1 on the [Ca2+ ]i,CaMKⅡ ? mRNA and protein expression of the SK-N-SH cells treated with chronic morphine and naloxone-precipitated withdrawal. Results ① Compared with control group,morphine significantly inhibited cell multiplication and resulted in calcium overload,and the expression of CaMKⅡ-? mRNA and protein noticeably increased ( P
