Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus.
- Author:
Amr E EL HAKIM
1
;
Yasser E SHAHEIN
;
Amira M ABOUELELLA
;
Mohamed E SELIM
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Boophilus annulatus; glycoproteins; larvae; vaccination
- MeSH: Amino Acid Sequence; Animals; Cattle; Cattle Diseases/immunology/*parasitology/prevention & control; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Glycoproteins/immunology/*isolation & purification; Immunoblotting; Isoelectric Focusing; Ixodidae/chemistry/*immunology; Male; Molecular Weight; Rabbits; Sequence Analysis, Protein; Tick Infestations/immunology/parasitology/prevention & control/*veterinary
- From:Journal of Veterinary Science 2007;8(2):175-180
- CountryRepublic of Korea
- Language:English
- Abstract: The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, Boophilus annulatus (Acari: Ixodidae). Two specific larval glycoproteins (GLPs) were isolated by two-step affinity chromatography. The larval immunogens were first purified with CNBr-Sepharose coupled to rabbit anti-larval immunoglobulins, and the glycoproteins were then purified with Con-A Sepharose. These glycoproteins have molecular weights of approximately 32 and 15 kDa with isoelectric points between 6.8 and 7.2. Antibodies against the two GLPs, labeled I and II, were detected in the anti-whole tick, -whole larval, and -gut antigens through immunoblot analysis. These results suggest that these GLPs are good immunogens and can be useful in the vaccination of cattle against tick infestation.
