Protective effects of astragaloside Ⅳ on hypertrophy induced by angiotensinⅡ in primary cultured cardiomyocytes of neonatal rats
- VernacularTitle:黄芪苷Ⅳ对血管紧张素Ⅱ诱导新生大鼠心肌细胞肥大的保护作用
- Author:
Hongmin ZHOU
;
Yuzhang JIN
;
Wenli XIE
;
Yanxia LIU
- Publication Type:Journal Article
- Keywords:
astragaloside Ⅳ;
angiotensinⅡ;
myocardial hypertrophy;
[Ca2+]i;
sarcoplasmic reticulum Ca2+-ATPases;
calcineurin;
primary culture
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effects and mechanism of astragaloside Ⅳ on hypertrophy induced by angiotensinⅡ(AngⅡ)in primary cultured cardiomyocytes of neonatal rats.Methods Cardiomyo-cytes were incubated with AngⅡ to establish the hypertrophy model of primary cultured cardiomyocytes in neonatal rats.AST 10 mg?L-1 or 20 mg?L-1 were added with AngⅡ simultaneously to observe its protective effects on cardiomyocytes.The diameter and the total protein content of cardiomyocyte were measured.The intracellular free calcium concentration([Ca2+]i),activity of sarcoplasmic reticulum Ca2+-ATPases(SERCA)and activity of calcineurin(CaN)were determined.Results Compared with control group,diameter and total protein content of cardiomyocyte induced by AngⅡ were increased significantly,which were inhibited by AST 10 mg?L-1 and 20 mg?L-1,respectively(P
