Effect of Tetrahydrozoline on the Permeability of Trabecular Meshwork Cell Monolayer.
10.3341/jkos.2017.58.1.69
- Author:
Seok Jin HWANG
1
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Permeability;
Survival;
Tetrahydrozoline;
Trabecular meshwork cells
- MeSH:
Adrenergic Agonists;
Humans;
Nitric Oxide;
Permeability*;
Trabecular Meshwork*
- From:Journal of the Korean Ophthalmological Society
2017;58(1):69-73
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effects of tetrahydrozoline (THZ) on the survival of cultured human trabecular meshwork cells (HTMC) and the permeability of HTMC monolayer. METHODS: Primary cultured HTMC were exposed to an adrenergic agonist (0.01, 0.1, 1.0 or 10 µM THZ) for 1 day and 3 days. Carboxyfluorescein permeability through the HTMC monolayer was measured using Transwell. Cellular viability and nitric oxide (NO) production were assessed using MTT and Griess assays, respectively. RESULTS: THZ did not affect the cellular survival (p > 0.05) or NO production (p > 0.05). THZ significantly increased the carboxyfluorescein permeability through the HTMC monolayer in a dose-dependent manner compared with non-exposed control (p < 0.05) after exposure for 1 and 3 days. CONCLUSIONS: THZ does not affect the survival of HTMC but decreases the permeability of HTMC monolayer in a dose-dependent manner. Thus, THZ may possibly decrease trabecular outflow.
