Effects of Tangshentang on the expression of LOX-1 and secretion of extracellular matrix induced by ox-LDL in rat masengial cells
- VernacularTitle:糖肾汤对氧化低密度脂蛋白诱导的系膜细胞血凝素样氧化低密度脂蛋白受体-1表达及细胞外基质分泌的影响
- Author:
Yueguang DU
;
Kefu CHAI
;
Tianxi ZHAO
- Publication Type:Journal Article
- Keywords:
Tangshentang;
ox-LDL;
LOX-1;
Kidney MC;
TGF-?1;
Extracellular matrix
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of Tangshentang on the expression of LOX-1 and secretion of TGF-?1,FN,ColⅣ in cultured rat masengial cells(RMCs) induced by ox-LDL and to explore its molecular mechanism of preventing and treating diabetic nephropathy.Methods: The cultured RMCs were divided into control group,ox-LDL group,Tangshentang group(respectively high,medium and low group)and rosiglitazone group.The mRNA expression was tested by RT-PCR.Synthesis of TGF-?1,FN,ColⅣ in cultured RMCs were determined by ELISA methods.Results: Tangshentang drug serum significantly attenuated up-regulation of TGF-?1,LOX-1 mRNA expression and synthesis of TGF-?1,FN,ColⅣ in a dose-dependent manner,with the peak at middle concentration,in RMCs stimulated by ox-LDL(50?g/ml).Conclusion: Tangshentang drug serum may protect kidney from injury by ox-LDL via the decreased expression of LOX-1 to reduce the uptake of ox-LDL and subsequently inhibiting TGF-?1 secretion,as well as the deposition of FN,ColⅣ.
