Studies on the apoptosis induction mechanism of andrographolide in human esophageal cancer Ec9706 cells
- VernacularTitle:穿心莲内酯诱导人食管癌Ec9706细胞凋亡机制研究
- Author:
Guifu DAI
;
Jin ZHAO
;
Qinduan WANG
;
Shijie MAO
;
Wei XIA
;
Hongmin LIU
- Publication Type:Journal Article
- Keywords:
andrographolide;
caspase activation;
Ec9706 cells;
bcl-2;
apoptosis
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the mechanism of andrographolide(AD) on the proliferation and apoptosis induction in human esophageal cancer Ec9706 cells.Methods The spectrometry was used to detect the activity of caspase-3 in human esophageal cancer Ec9706 cells treated with or without AD for 6 h,12 h and 18 h,and to detect the activity of caspase-8 and caspase-9 in human esophageal cancer Ec9706 cells treated with or without AD for 6 h.The influence of AD on the proliferation of Ec9706 cells after treatment with or without Z-VAD-FMK(a broad-spectrum caspase inhibitor) was determined by MTT method and the result was compared.The changes of gene expression levels of bcl-2 were determined by immunohistochemical method.Results The expression level of bcl-2 gene was obviously lower in the cells treated with AD(30 mg?L-1,P
