Protective effects of silymarin on fumonisin B1-induced hepatotoxicity in mice.
- Author:
Mahmut SOZMEN
1
;
Alparslan Kadir DEVRIM
;
Recai TUNCA
;
Murat BAYEZIT
;
Serpil DAG
;
Dinc ESSIZ
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: caspase-8; fumonisin B1; fibroblast growth factor-2; galectin-3; silymarin
- MeSH: Animals; Antioxidants/*pharmacology; Apoptosis/drug effects; Cell Proliferation/drug effects; Female; Fibroblast Growth Factor 2/genetics/metabolism; Fumonisins/*toxicity; Gene Expression Regulation/drug effects; Hepatocytes/*drug effects; Ki-67 Antigen/metabolism; Liver/drug effects; Mice; Mice, Inbred BALB C; Mycotoxins/*toxicity; Neovascularization, Physiologic/drug effects; Proliferating Cell Nuclear Antigen/metabolism; Silymarin/*pharmacology; Tumor Necrosis Factor-alpha/metabolism; Vascular Endothelial Growth Factor A/genetics/metabolism
- From:Journal of Veterinary Science 2014;15(1):51-60
- CountryRepublic of Korea
- Language:English
- Abstract: The present study was conducted to investigate the effect of silymarin on experimental liver toxication induced by Fumonisin B1 (FB1) in BALB/c mice. The mice were divided into six groups (n = 15). Group 1 served as the control. Group 2 was the silymarin control (100 mg/kg by gavage). Groups 3 and 4 were treated with FB1 (Group 3, 1.5 mg/kg FB1, intraperitoneally; and Group 4, 4.5 mg/kg FB1). Group 5 received FB1 (1.5 mg/kg) and silymarin (100 mg/kg), and Group 6 was given a higher dose of FB1 (4.5 mg/kg FB1) with silymarin (100 mg/kg). Silymarin treatment significantly decreased (p < 0.0001) the apoptotic rate. FB1 administration significantly increased (p < 0.0001) proliferating cell nuclear antigen and Ki-67 expression. Furthermore, FB1 elevated the levels of caspase-8 and tumor necrosis factor-alpha mediators while silymarin significantly reduced (p < 0.0001) the expression of these factors. Vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2) expressions were significantly elevated in Group 4 (p < 0.0001). Silymarin administration alleviated increased VEGF and FGF-2 expression levels (p < 0.0001). In conclusion, silymarin ameliorated toxic liver damage caused by FB1 in BALB/c mice.
