Effects of advanced glycation end-products on expression of acyl coenzyme A:cholesterol acyltransferase-1 in THP-1 macrophages
- VernacularTitle:糖基化终产物对THP-1巨噬细胞酰基辅酶A:胆固醇酰基转移酶-1表达的影响
- Author:
Qihong YANG
;
Qiang XU
;
Hong ZHANG
;
Liangyi SI
- Publication Type:Journal Article
- Keywords:
diabetes;
advanced glycation end products;
acyl coenzyme A: cholesterol acyltransferase-1;
gene expression
- From:Journal of Third Military Medical University
2003;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of advanced glycosylation end-products (AGEs) on the expression of acyl coenzyme A: cholesterol acyltransferase-1 (ACAT-1) in cultured THP-1 macrophages. Methods THP-1 cells were differentiated into macrophages after cultured in RPMI1640 media containing 0.1 ?mol/L PMA for 72 h. THP-1 macrophages were then exposed to AGE-modified bovine serum albumin (AGE-BSA, at concentration of 50, 100, 200 mg/L) for 24 h or to 200 mg/L AGE-BSA for 0, 12, 24, 36 h. Expression of ACAT-1 mRNA and protein in THP-1 macrophages was measured by RT-PCR and Western blot, respectively. Results After induced by 0.1 ?mol/L PMA, THP-1 cells stopped proliferation and differentiated into macrophages. Treatment of THP-1 macrophages with AGE-BSA resulted in an increase in the mRNA and protein levels for ACAT-1 in a dose-and time-dependent manner. Conclusion AGEs upregulate the expression levels of mRNA and protein for ACAT-1 in cultured THP-1 macrophages, which might be partly involved in the atherogenesis in diabetic patients.
