Effect of new QingKaiLing injection on adhesion molecules expression of an in vitro ischemia and reperfusion injury model of rat brain microvascular endothelial cell
- VernacularTitle:新清开灵注射液对大鼠脑缺血再灌注损伤微血管内皮细胞黏附分子表达的影响
- Author:
Yonghong GAO
;
Zhengzhong YUAN
;
Fuling NIU
;
Lingqun ZHU
;
Pengtao LI
;
Shuoren WANG
- Publication Type:Journal Article
- Keywords:
Ischemia and reperfusion;
Brain microvascular endothelial cell;
Adhesion molecules;
New QingKaiLing injection
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the expression of cell adhesion molecules ICAM-1 and VCAM-1of cultured rat brain microvascular endothelial cells(MVEC),expecting to explore the mechanisms of new QingKaiLing injection protecting brain from injury of inflammatory cascade in cerebral ischemia diseases.Methods: Rat cerebral MVEC were extracted by separating microvessel sections and collagenase enzymatic digesting,an in vitro ischemia reperfusion model was established(Kreb,95%N2+5%CO2),the protein and mRNA expression of ICAM-1 and VCAM-1 were detected by using immunocytochemical stain and RT-PCR method.Results:The expression of adhesion molecules of model group were significantly higher than those of noral group(P
