Inhibitive effect of specific stealth siRNAs on TGF-?_1 expression of mouse lung fibroblasts
- VernacularTitle:特异性stealth siRNA抑制小鼠肺成纤维细胞TGF-?_1表达的实验研究
- Author:
Weitao YU
;
Shehuai CUI
;
Xuemei YANG
- Publication Type:Journal Article
- Keywords:
stealth siRNA;
TGF-?_1;
lung fibroblast
- From:Journal of Third Military Medical University
2003;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To design and produce specific stealth siRNAs and detect their inhibitive effects on TGF-?1 expression.Methods Three stealth siRNAs aimed directly at different sequences(stealth-48,stealth-166,stealth-594) in TGF-?1 mRNA were made.These siRNAs were transfected into BALB/c mouse lung fibroblast in vitro,then the TGF-?1 expression in those lung fibroblasts was detected by immunohistochemistry.Results In different time periods,the TGF-?1 expression was differently depressed by three stealth siRNAs in vitro.The inhibitory effects of stealth-166 was better than the other two stealth siRNAs.The inhibition could be detected in 48 h,reached the highest level in 72 h and began to attenuate 96 h later.Conclusion The TGF-?1 expression of mouse lung fibroblasts in vitro can be depressed by synthetic stealth siRNAs.It seems possible to provide a new way for the treatment of pulmonary interstitial fibrosis.
