Effects and mechanisms of ATRA on proliferation,cell cycle of lung carcinoma cell line A549
- VernacularTitle:ATRA对A549人肺腺癌细胞株增殖、细胞周期的影响及其机制研究
- Author:
Renjie ZHOU
;
Weigong LIAO
;
Zhenzhou YANG
;
Jiaxin MIN
;
Yingbin XIAO
- Publication Type:Journal Article
- Keywords:
all-trans retinoic acid;
lung cancer;
proliferation;
cell cycle
- From:Journal of Third Military Medical University
2003;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and mechanisms of all-trans retinoic acid(ATRA)on the proliferation and cell cycle of lung carcinoma cell line A549.Methods The A549 cells were treated with ATRA at the dosages of 5,10,50 ?mol/L for 1-7 d.The proliferation of A549 was assessed by MTT method and cell cycle was analyzed by flow cytometry.The expressions of CDK4,Rb and p-ERK1/2 were assessed by Western blotting.CyclinD1 mRNA was analyzed by SYBR-PCR amplification.Results ATRA obviously inhibited the proliferation of A549 cells,and the cell cycle was arrested in G0/G1 phase.The expression of p-ERK1/2 protein and CyclinD1 mRNA on A549 cells were decreased.Conclusion ATRA might inhibit the proliferation of A549 cells through down-regulating p-ERK1/2 protein and CyclinD1 mRNA.
