Feasibility of MSCs mobilization by G-CSF and its prosthetic effect in traumatic brain injury
- VernacularTitle:G-CSF动员循环间充质干细胞及其对小鼠颅脑损伤修复的可行性分析
- Author:
Jun DENG
;
Guoping AI
;
Taoli ZHOU
;
Junping WANG
;
Hui XU
;
Zhongmin ZOU
;
Shiwu DONG
;
Lei HAO
;
Xinze RAN
;
Yongping SU
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cell;
trauma;
mobilization;
G-CSF
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of mobilization circulating MSCs by G-CSF and observe the repairing effect of G-CSF mobilization in severe mouse traumatic brain injury(TBI) model.Methods MSCs-derived bone marrow and peripheral blood(PB) were cultured and its CFU-F were counted after mobilization by G-CSF.At 2,24,48,96,120,144,192,264,336 h after severe TBI in mice was establish,the neurobehavior of mice was measured by neurological examination and motor functional test,and mortality rate and pathologic changes were analyzed.Results MSCs-derived PB were successfully cultured.The CFU-F of mobilization group increased significantly than that of control group(P
