Effects of rapid eye movement sleep deprivation on cognitive function and expression of brain-derived neurotrophic factor in rat hippocampus
- VernacularTitle:快速眼球转动期睡眠剥夺对大鼠学习、记忆能力及其海马组织脑源性神经营养因子表达的影响
- Author:
Chenjing YE
;
Zhongxin ZHAO
- Publication Type:Journal Article
- Keywords:
sleep deprivation;
hippocampus;
brain-derived neurotrophic factor;
learning;
memory
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of various degrees of rapid eye movement (REM) sleep deprivation (RSD) and sleep recovery on cognitive function (learning and memory) and expression of brain-derived neurotrophic factor (BDNF) in the rat hippocampus. Methods: Male SD rats were divided into 8 groups (n=12): blank control group (with normal sleep), environmental control, RSD 1 d, RSD 3 d, RSD 5 d, RSD 7 d, recover sleep 6 h after 7 d RSD (RS 6 h), and recover sleep 12 h after 7 d RSD (RS 12 h). The modified multiple platform method (MMPM) was used to establish sleep deprivation model in rats. The cognitive functions of rats were tested by Y-type maze. The hippocampal BDNF mRNA and protein levels were detected by real-time PCR and immunohistochemical method. Results: The failure reaction times of all RSD groups and the 2 RS groups were significantly more than those in control group and environmental control group (P
