Preparation of biodegradable microspheres of Schistosoma japonicum recombinant glutathione-S-transferase
- VernacularTitle:日本血吸虫重组谷胱甘肽转移酶可生物降解微球的制备
- Author:
Pingping ZHOU
;
Xiaoniu TANG
;
Shaosheng WANG
;
Wenkui CHEN
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Glutathione-S-transferase;
Recombinant;
Microsphere
- From:
Chinese Journal of Schistosomiasis Control
1989;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare biodegradable microspheres of Schistosoma japonicum recombinant glutathione-S-transferase. Methods By using the constructed expression plasmid pET28a(+)-SjGST, fusion protein of Schistosoma japonicum recombinant glutathione-S-transferases were expressed in LB by E.coli BL21,rSjGST fusion protein was purified through column of resin,and then the fusion protein concentration was detected; by using an oil-in-oil emulsion-solvent extraction method, the rSjGST biodegradable microspheres were prepared in PLGA (75/25).The properties of microspheres including shape、diameter、diameter’s distribution and span were assayed under microscope. Results The concentration of protein after purification was 8.323 mg/ml, and the microspheres were spherical and showed normal distribution.The diameter was (7.3?1.2) ?m,the span was 0.52,the load of the rSjGST was 7.62%. Conclusion Biodegradable microspheres of Schistosoma japonicum recombinant glutathione-S-transferase are successfully prepared, which provides the basis for further studies on protective immunity of mice.
