The changes of phosphodiesterase-4 activity in rat acute lung injury
- VernacularTitle:大鼠急性肺损伤模型肺组织中磷酸二酯酶4活性的变化
- Author:
Xuyang ZHENG
;
Qiangmin XIE
;
Xiaogang DU
;
Hui ZHANG
;
Jiqiang CHEN
- Publication Type:Journal Article
- Keywords:
acute lung injury(ALI);
phosphodiesterase 4(PDE4);
lipopolysacdharide(LPS);
tumor necrosis factor(TNF-?);
neutrophil
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the changes of phosphodiesterase4(PDE4,type 4 cAMP-specific PDE) activity,TNF-? and neutrophil recruitment in experimental rat lung injury(ALI).Methods ALI in the rat was induced by lipopolysacdharide(LPS).PDE4 activity was measured with HPLC,and the level of TNF-? was detected with ELISA,neutrophil infiltration in bronchoalveolar lavage fluid(BALF) and lung tissues was detected by cell count and morphological analysis.Result Lung tissue PDE4 activity significantly increased as early as 1 h,peaked 6 h,and then markedly lowered at 24 h after intratracheal administration of LPS,while there was a same time-course change of total white cell and neutrophil in the BALF(r=0.83,P
