Determination of Two Triterpene Acids in Acantopanax Senticosus (Rupr.et Maxim.) Harms by HPLC
- VernacularTitle:HPLC法测定刺五加中两种三萜酸成分的含量
- Author:
Shengqin ZOU
;
Yulun HUANG
- Publication Type:Journal Article
- Keywords:
Acantopanax senticosus (Rupr. et Maxim.) Harms;
ursolic acid;
oleanolic acid;
HPLC;
content determination
- From:
Journal of Zhejiang Chinese Medical University
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] Ursolic acid and oleanolic acid in Acantopanax senticosus (Rupr. et Maxim.) Harms were extracted with the ultrasonic technology. A determination method of ursolic acid and oleanolic acid was established with the high performance liquid chromatograph and photodiode array detector. [Method]The chromatographic column, Nova-Pak C18 (3.9?300 mm, 4 ?m), methanol-water (88∶12,V/V), mobile phase with 0.8 mL/min flow rate, the detected wave length (210nm),and the column temperature(25 ℃) were adopted. [Results] The calibration curve of ursolic acid was linear under the content of 0.136 ~1.224 ?g and the correlation coefficient was 0.9995. The calibration curve of oleanolic acid was linear under the content of 0.052 ~0.468 ?g and the correlation coefficient was 0.9994. The average recovery of ursolic acid was 98.1 % and RSD was 1.1 % (n=5). The average recovery of oleanolic acid was 97.5 % and RSD was 1.0 % (n=5). [Conclusion]The content determination of HPLC is accurate and reliable, and can be used to analyse quantificationally ursolic acid and oleanolic acid in Acantopanax senticosus (Rupr. et Maxim.) Harms.
