Curcumin causes histone acetylation enhancement in Raji,HL-60 and K562 cell lines
- VernacularTitle:姜黄素诱导Raji、HL-60和K562组蛋白乙酰化的研究
- Author:
Yan WANG
;
Junbin HU
;
Yan CHEN
;
Guohui CUI
- Publication Type:Journal Article
- Keywords:
curcumin;
histone;
acetylation;
deacetylation
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate whether the mechanism of curcumin antagonizing tumor correlates with histone acetylation/deacetylation,which is regarded as modulation of transcription level.Methods Measure the survival rates of Raji by treatment with various concentration of curcumin or TSA by the method of MTT;Measure the expression of acetylated histone_3 in Raji,HL60 and K562 when treated with various concenration of curcumin or TSA by means of Immunohistochemistry and FACS.Results Curcumin could inhibit Raji cell proliferation in a time-dependent and dose-dependent manner.Curcumin at the concertration of 25 ?mol?L~(-1) could enhance acetylated histones in the three tumor cells(P
