Effect of caffeic acid phenethyl ester on the expression of ?-catenin in the cultured human colorectal cancer cell lines
- VernacularTitle:咖啡酸苯乙酯对大肠癌细胞?-catenin蛋白表达的影响
- Author:
Debing XIANG
;
Yujun HE
;
Jianghong MOU
;
Dong WANG
;
Zengpeng LI
;
Hualiang XIAO
;
Qinhon ZHANG
- Publication Type:Journal Article
- Keywords:
colorectal cancer;
?-catenin;
caffeic acid phenethyl ester
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of caffeic acid phenethyl ester (CAPE) on the expression of ?-catenin in the cultured colorectal cancer cell lines. Methods HCT116 and W480 cells were treated with CAPE at serial concentrations of 2.5, 5, and 10 mg/L. ?-catenin protein expression was assayed by Western blot analysis. ?-catenin localization was detected by indirect immunofluorescence. Results CAPE treatment was associated with decreased total ?-catenin protein expression. The expression of ?-catenin at the cell nucleus and cytoplasm was downregulated, but at the cell-cell linked site the ?-catenin protein expression was upregulated. Conclusion CAPE can downregulate the expression of ?-catenin and inhibit the translocation of ?-catenin to nucleus, which may play an important role in the anticancer activity of CAPE.
