Modification and identification of tyrosinase related protein-2(180-188) epitope
- VernacularTitle:酪氨酸激酶相关蛋白-2_(180-188)表位的修饰和初步鉴定
- Author:
Yan TANG
;
Yuzhang WU
;
Zhihua LIN
;
Jing WEI
;
Huimin WANG
- Publication Type:Journal Article
- Keywords:
tyrosinase related protein-2;
altered peptide ligand;
modification;
identification
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To modify HLA-A2.1 restricted epitope of tyrosinase related protein-2 (TRP-2) (180-188) of human melanoma differentiation antigen and enhance HLA-A2.1 molecule its affinity to. Methods The TRP-2 (180-188) nave epitope was altered with the predominant amino acid for the primary anchor residues and auxiliary anchor residues. Altered peptide ligands (APLs) were scanned by polynomial method, the quantitative motif method and OSAR methods. The analogues their affinity to HLA-A2.1 molecule were assayed. Results Compared with the nave peptide, APLs had stronger affinity to HLA-A2 molecules. Conclusion Our results suggest that altered nave epitope with P1 (Y) and/or P2(L, M) can enhance affinity to HLA-A2 molecule. However, the immunogical efficacy of APLs needs to be identified in studies in vitro and in vivo.
