Establishment and characterization of a cisplatin induced multidrug-resistant human small cell lung cancer cell subline
- VernacularTitle:顺铂诱导人小细胞肺癌多药耐药细胞系NCI-H446/CDDP的建立及其生物学特征分析
- Author:
Pin QIAN
;
Fuyun JI
;
Guisheng QIAN
- Publication Type:Journal Article
- Keywords:
carcinoma, small cell lung;
drug resistance, multiple;
cisplatin
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a multidrug-resistant cell subline NCI-H446/CDDP of human small cell lung cancer and characterize its biological properties. Methods The cell subline NCI-H446/CDDP was derived from human small cell lung cancer cell line NCI-H446 by exposing it to high concentration first followed by being cultured with gradually increasing dose of cisplatin, which used to be the first-line chemotherapeutic drug for lung cancer. The cell growth curve and the doubling time were determined by cell counting. The chemosensitivities of NCI-H446/CDDP and NCI-H446 to cisplatin, hydroxycamptothecine, vincristin, 5-fluorouracil and topotecan were tested and IC 50 measured by MTT. Changes in cellular morphology and ultrastructure were observed under inverted-microscope, scanning electron microscope and transmission electron microscope, respectively. Results Multidrug-resistant cell subline (NCI-H446/CDDP) of human small cell lung cancer was established successfully after culturing NCI-H446 in a high concentration of cisplatin first, followed by subjecting it to increasing concentrations of CDDP until they could stably grow in the culture medium containing 0.5?g/mL CDDP. The rate of cell proliferation of NCI-H446/CDDP was similar to that of NCI-H446, but the number of the former cells exhibiting S phase increased (20.24% vs 18.42% P
