Shikonin induce HeLa cell apoptosis via a caspase-dependent mechanism
- VernacularTitle:紫草素诱导HeLa细胞凋亡经过caspase激活的机制
- Author:
Zhen WU
;
Lijun WU
;
Shinichi TASHIRO
;
Satoshi ONODERA
;
Takash IKEJIMA
- Publication Type:Journal Article
- Keywords:
shikonin;
HeLa cell;
apoptosis;
caspase;
anti-tumor activity
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the mechanisms of shikonin-induced HeLa cell apoptosis. METHODS Cytotoxicity assay by MTT, DNA fragmentation assay, LDH activity-based cytotoxicity assay and Western blot analysis were carried out. RESULTS Shikonin inhibited HeLa cell growth in a dose- and time-dependent manner. Apoptotic cell death was observed within 24 h after 40 ?mol?L -1 shikonin treatment. Shikonin induced HeLa cell apoptosis involved in the activation of caspase-3 and caspase-8, which is similar to the Fas or TNF-? signaling transduction pathways. CONCLUSION Shikonin induced caspase-dependent apoptosis in HeLa cells.
