Enrichment and purification process of total flavonoids from Scutellaria baicalensis with macroporous resins
- VernacularTitle:大孔树脂吸附法对黄芩总黄酮富集纯化工艺的研究
- Author:
Binguo LIU
;
Lei ZHONG
;
Wenyong GUO
;
Heming MI
- Publication Type:Journal Article
- Keywords:
macroporous adsorption resin;
Scutellaria baicalensis Georgi;
baicalin;
total flavonoids;
orthogonal design
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the suitable condition for enrichment and purification of total flavonoids from Scutel-laria baicalensis with macroporous resins and to select the optimal parameters. Methods: Using the total flavonoids as the standard, the optimum macroporous resins were selected. Sample concentration, adsorption duration and alcohol concentration were investigated by orthogonal experiment design of form L9(34). Results: The 1300 resin was the suitable one for enriching and purifying total flavonoids. The optimum conditions Were as follows :adsorption time at 40 min, sample concentration at 4. 00 mg/ml (pH 5. 0) ,and 30% alcohol as the elution reagent. Conclusion: 1300 resin can be used to enrich and purify the total flavonoids of Scutellaria baicalensis, which can increase the rate of enrichment.
