EXPERIMENTAL STUDY ON CULTURE AND DIFFERENTIATION OF BMSC FROM RHESUS MONKEY
- VernacularTitle:恒河猴骨髓基质细胞体外诱导分化成神经干细胞的实验研究
- Author:
Wei WANG
;
Xiaodan JIANG
;
Ruxian XU
- Publication Type:Journal Article
- Keywords:
bone marrow stromal cells;
stem cell;
cell culture;
rhesus monkey
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
To study the growth, expansion and differentiation of bone marrow stromal cells (BMSC) of rhesus monkey, the BMSC were isolated from adult rhesus monkeys, cultured and induced with a special medium confected in our lab. Identification was performed with immunochemichemistry method. The results showed that BMSC could proliferate and generate clone when culturing in vitro. These cells grow rapidly and differentiated into neuron like cells and astrocyte like cells. The cell clones proliferated from stem cell could express Nestin antigen and the differentiated cells expressing GFAP or NSE antigen respectively. In this study, we did not observe RA, BFGF and EGF remarkably influencing the induction and differentiation of the BMSC. It is suggested that the BMSC from rhesus monkey have the self renewal and differentiation abilities. They might differentiate into neuron like and astrocyte like cells which express GFAP or NSE. As available easily, the BMSC could be considered as the ideal seed cells of the neural stem cells.
