Effects of AGE-?_2m on synovial cell adhesion, spreading and proliferation
- VernacularTitle:晚期糖基化终产物修饰的?_2微球蛋白对人滑膜细胞黏附、伸展和增殖的影响
- Author:
Weihua PENG
;
Fanfan HOU
;
Xun ZHANG
;
Al AT
- Publication Type:Journal Article
- Keywords:
?2-microglobulin;
Advanced glycation end products;
Synovial cell;
Dialysis-related amyloidosis
- From:
Chinese Journal of Nephrology
1997;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test the hypothesis that attachment of synovial cell to &?2-microglobulin modified with advanced glycation end products (ACE-?2m) would affect cell adhesion, spreading and proliferation.Methods Normal human synovial cells (type B cells) were isolated and plated in culture dishes coated with AGE-?2m or with normal extracellular matrix proteins (EMP). Adhesion was analyzed by counting the isotope-labelled cells. Spreading was tested using a light microscope and proliferation determined by 3H-TdR incorporation and counting the number of cells. Results Synovial cells adhered less effectively to AGE-?2m, ?2m and AGE-collagen than to the normal EMP (collagen and fibronectin). Cells interacting with AGE-?2m, ?2m or AGE-collagen also demonstrated less extensive spreading throughout the examined time intervals (60-120 minutes after plating), and decreased 3H-TdR incorporation and cell numbers after 72 hours of plating when compared to cells interacting with normal EMP. Conchusion AGE-?2m in amyloid may alter synovial cell behavior in situ in ways which cods contribute to the development of dialysis-related amyloidodsis(DRA).
