High Expressioii of Erythropoktin (EPO) cDNA in COS7 Cells
- VernacularTitle:人红细胞生成素cDNA在COS7细胞中高效表达
- Author:
Yunxu CAO
;
Xiangjun ZHOU
;
Yong TONG
;
Yonghua LOU
;
Zhaorong WEN
;
Deru LU
- Publication Type:Journal Article
- Keywords:
erythropoietin;
mutagenesis. gene transfer
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
An recombinant vector pCSV-EPO for expression of EPO cDNA in mammalian cells was constructed by the techniques of gene recombinant, PCR amplification and region-specific mutagenesis. The pCSV-EPO was introduced into COS7 cells by DEAE-dextran-mediated transfection. The expression of the EPO was demonstrated by EPO-ELISA assay. At 48h post transfection, the EPO level was 25ng/ml and 72 h was 17ng/ml.
