The effect of ( - )Daurisoline on cytoplasmic free calcium concentration of rabbit lymphocytes
- VernacularTitle:(一)蝙蝠葛苏林碱对兔淋巴细胞内钙含量的影响
- Author:
Jinxuan CHENG
;
Yibin ZHANG
;
Jinhong DUAN
- Publication Type:Journal Article
- Keywords:
(-)daurisoline;
lymphocytes;
calcium;
fluorescent indicator;
fu-ra -2/Am
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
This paper reported the estimation of free calcium concentration in rabbit resting lymphocytes, and the effect of (-) Daurisoline (8701) on the cyto-plasmic free calcium concentration by using fluorescent indicator Fura -2/Am estimating technique. Results were compared with the effect of the Ca2-ionophore A23187.The results indicated that the free calcium concentration of resting lymphocytes was 109. 6nmol ? L-1, after 10-5 mol? L-1,10-4.5 mol ? L-1,and 10-4 mol ? L-1-)Daurisoline administration, the cyto-plasmic free calcium concentration increased to 178. 8%, 204. 4%and 817. 5% respectively, while same concentration A23187 only led the increase of 164. 6% , 198.4% and 327% respectively.Results suggested that ( - )Daurisoline may be a Ca2- agonist and may induce the cytotoxicity effect on lymphocytes at high concentr ation.
