A COMPARISON OF ENZYMATIC ACTIVITIES, Ca~(2+) BINDING AND Ca~(2+) TRANSPORT FUNCTIONS IN SUBCELLULAR MEMBRANE FRACTIONS ISOLATED FROM DOG MESENTERIC ARTERIES AND VEINS
- VernacularTitle:狗肠系膜动静脉平滑肌的亚细胞膜酶活性和钙—累积功能之比较
- Author:
Yongyuan GUAN
;
Anguo SHI
;
Chiuyin KWAN
- Publication Type:Journal Article
- Keywords:
Mesenteric artery;
Vein;
Subcellular membrane;
Ca~(2+)-binding;
Ca~(2+) -transport;
Membrane marker enzyme
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Using subcellular membrane fractions isolated from smooth muscles of dog mesenteric arteries and veins, we compared their enzymatic activities, Ca2+ bindind and Ca2+ transport. The micro-somal (MIC)and plasma membrane(F2) fractions were similarly enriched 4-5 and 10-12 fold respectively in the plasma membrane marker enzymes over the starting membrane mixture. However, MIC and F2 fractions from venous smooth muscle showed higher Mg2+-ATPase activity, slightly increased Ca2+ binding and reduced Ca2+-transport compared to the corresponding fractions from arteries.
