Study on the apoptosis of T lymphocytes of peripheral blood in systemic lupus erythematosus(SLE) and the correlated pathogenesis
- VernacularTitle:SLE患者外周血T细胞亚群凋亡特点及其相关机制的研究
- Author:
Xiaole WANG
;
Chaolin QIU
;
Xiumei WU
;
Xiaohui JI
;
Dongqing ZHANG
- Publication Type:Journal Article
- Keywords:
Systemic lupus erythematosus(SLE);
T lymphocytes;
Apoptosis;
IL-10;
Fas;
FasL
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the apoptosis of T lymphocyte subsets of peripheral blood in systemic lupus erythematosus(SLE) and the correlated pathogenesis.Methods:The percentages of T lymphocyte subsets,the positive expression rates of Fas/FasL on T lymphocyte subsets,and the apoptosis of T lymphocyte subsets were determined by three-colorflow cytometry.The level of serum IL-10 was analyzed by ABC-ELISA.IL-10 antibody and FasL antibody were added respectively into the 48 hours' culture of 10 SLE patients' PBMCs in vitro,whose serum IL-10 significantly increased,and then,the changes of T lymphocyte subsets of SLE PBMCs were determined by three-colorflow cytometry.Results:1) In SLE,mainly in hyperactive SLE,the apoptosis of CD4+ T lymphocytes increased significantly(P
