HPLC determination of mycophenolic acid in serum of early period of renal transplant patients
- VernacularTitle:高效液相色谱法测定早期肾移植患者麦考酚酸的血药浓度
- Author:
Weihua DONG
;
Yalin DONG
;
Xin ZHENG
;
Haisheng YOU
- Publication Type:Journal Article
- Keywords:
renal transplantation;
mycophenolate mofetil;
mycophenolic acid;
high-performance liquid chromatography(HPLC);
blood concentration
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high-performance liquid chromatography method for determination of mycophenolic acid in human plasma.Methods The method was performed with C18 column(3.9mm?300mm).The mobile phase consisted of 40mmoL/L TBA solution-acetonitrile adjusted to pH 4 with orthophosphoric acid(55∶45,V∶V),with a flow rate 0.8mL/min.The UV detection wavelength was 254nm.Plasma samples were extracted with methanol.Results The linear concentration of the calibration curve ranged from 0.8 to 51.45mg/L(r=0.9998).The relative recoveries were between 95.5% and 109.2%,and the extraction recoveries were more than 90%.Relative standard deviation(RSD) of intraday and interday assays were both less than 10%.Conclusion The method is proved to be reliable and simple for monitoring mycophenolic acid in human plasma and bioequivalence research.
