Studies on B cell differentiation from cord blood CD34~+CD19~- hematopoietic stem/progenitor cells in vitro
- VernacularTitle:脐血CD34~+CD19~-造血干/祖细胞体外B细胞分化条件研究
- Author:
Jiabo HU
;
Quanhui MA
;
Fei MAO
;
Wenrong XU
;
Huaxi XU
- Publication Type:Journal Article
- Keywords:
Cord blood;
Hematopoietic stem cells;
B cells;
cell differentiation;
T3
- From:
Chinese Journal of Immunology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the condition of B cell differentiation from cord blood CD34+CD19-hematopoietic stem/progenitor cells in vitro.Methods:CD34+CD19-cells from cord blood were isolated and purified by using immunomagnetic beads separation system.CD34+CD19-cells supported by murine S-17 stromal cells were stimulated in co-culture with T3 and cytokines.Differentiated B cells were analyzed by flow cytometry.Results:The amplification of the B cells derived from CD34+CD19-hematopoietic stem/progenitor cells in co-culture with T3 and IL-7 reached 198-fold of increase,most of the induced cells expressed CD10 and CD19.Conclusion:In the experimental conditions selected,co-culture of CD34+CD19-cells with T3,IL-7 and murine S-17 stromal cells could stimulate differentiate toward to B cells in vitro.
