Effects of Lanthanum on Cell Multiplication and Intracellular Free Ca~(2+) Concentration in Primary Cultured Astrocytes of Rats
- VernacularTitle:镧对星形胶质细胞增殖能力和细胞内钙浓度的影响
- Author:
Shuai LU
;
Qiufang LIU
;
Ming QI
- Publication Type:Journal Article
- Keywords:
Rare earth;
Lanthanum;
Astrocyte;
Cell culture;
Calcium
- From:
Journal of Environment and Health
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of lanthanum on cell multiplication and intracellular Ca2+([Ca2+]i) concentration in primary cultured astrocytes of rats.Methods The primary cultured astrocytes of rats were exposed to 0.25,0.5,1.0 and 2.0 mmol/L LaCl3 for 24 h and 48 h.The cell multiplication was measured by MTT;[Ca2+]i concentration was determined by the fluorescent Ca2+ indicator Fura-2/AM.Results After astrocytes were incubated with 0.25,0.5,1.0 and 2.0 mmol/L LaCl3 for 24 h and 48 h,the survival rate of astrocytes was 86.24% and 82.99%,82.26% and 79.14%,52.32% and 49.97%,38.04% and 35.15% respectively,which was significantly decreased compared with the control group(P
