The effect on proliferation and apoptosis by Nucleostemin gene specific RNA interference in gastric-carcinoma cells
- VernacularTitle:Nucleostemin基因特异性RNA干扰对胃癌细胞增殖和凋亡的影响
- Author:
Xuezhi ZHENG
;
Jing HU
;
Wei SUN
;
Guilian LIU
;
Xiaoni LIU
;
Ziwei CAI
- Publication Type:Journal Article
- Keywords:
RNA interference;
nucleostemin;
gastric carcinoma;
cell proliferation;
apoptosis
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect on proliferation and apoptosis by RNA interference to inhibit Nucleostemin(NS) gene expression in gastric carcinoma SGC-7901 cells.Methods The NS-siRNA expression vector was transfected into gastric-carcinoma cells with LipofectamineTM2000 reagent.Then we detected the cell proliferation inhibition ratio by MTT assay,the levels of NS gene expression in all groups by RT-PCR,cell cycle by flow cytometry,cell apoptosis ratio by Annexin-V-FITC/PI kit.Results Compared with that in the control group,cell proliferation in S group decreased;at 24,48 and 72 h the cell proliferation inhibition ratio was 53.21%,71.54% and 87.47%,respectively,the level of NS gene expression reduced in S group.G0/G1 phase cell was 58.34%,S phase cell was 20.68%,and the cell apoptosis was 26.85%.Conclusion RNA interference could substantially inhibit NS gene expression in gastric carcinoma SGC-7901 cells,decrease cell proliferation,arrest cell cycle and increase apoptosis ratio.
