Designation of gACE epitope and preparation of its polgclonal antibody
- VernacularTitle:人gACE多肽抗原的设计及多克隆抗体的制备和鉴定
- Author:
Liping AN
;
Xiaoying WANG
;
Xiao HAN
;
Guangyu XU
;
Beiying AN
;
Song YANG
;
Peige DU
- Publication Type:Journal Article
- Keywords:
gACE;
Bioinformatics;
Polyclonal antibody
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare gACE polyclonal antibody for functional study of gACE.Methods:According to the bioinformatics analysis and prediction of the possible conformational structure,hydrophobicity and antigenicity of gACE and the principal for antibody production,a partial peptide with 18-amino acid residues of gACE was synthesized after homology search.The synthesized peptide was then used to immunize after coupling with KLH.The properties of anti-gACE were analyzed by ELISA,Western blot and immunohistochemistry.Results:The antigenicity was repredicted by bioinformatics analysis.The polyclonal antibody against gACE was successfully obtained and its specificity and sensitivity we conformed by ELISA,Western blot and immunohistochemistry.Conclusion:By the bioinformatics analysis and prediction,the hydrophilicity and antigenicity of gACE are analyzed.The antibody of gACE is successfully obtained.
