Apoptosis mechanism of cultured human hepatocyte HL-7702 induced by glycodeoxycholate
- VernacularTitle:甘氨脱氧胆酸盐诱导人正常肝细胞HL-7702凋亡及其机制的研究
- Author:
Liangmin CHUAN
;
Hua HONG
;
Wenfang HUANG
;
Yongchang YANG
;
Shaoqin RAO
;
Jun DENG
- Publication Type:Journal Article
- Keywords:
glycodeoxycholate;
cellular apoptosis;
\[Ca2+\]i;
Bcl-2;
Bax;
hepatocyte
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the apoptosis of cultured normal human hepatocyte HL-7702 cells induced by glycodeoxycholate(GCDC)and to explore its possible mechanism.Methods HL-7702 cells were incubated with various concentrations(100,150,200 and 250 ?mol/L)of GCDC.The changes of cellular morphology were observed under optical microscope.The apoptosis rate of HL-7702 was determined by Annexin V-FITC/PI double staining.The changes of HL-7702 cell intracelluar \[Ca2+\]i were determined with Fluo-3/AM load technique.The mRNA expression levels of Bcl-2/Bax in HL-7702 cells were analyzed by RT-PCR.Results Typical apoptotic morphological changes were observed after HL-7702 cells had been treated with 150 ?mol/L GCDC for 24 h;HL-7702 cells could be induced to undergo apoptosis in a concentration-dependent manner after 100,150,200,and 250 ?mol/L GCDC treatment for 24 hours.The apoptosis rates were(13.16?2.9)%,(20.3?3.0)%,(25.02?2.1)% and(45.02?3.5)%,which were markedly higher(P
