His-tag does not change immunogenicity of recombinant protein G1F/M2 of respiratory syncytial virus
- VernacularTitle:His-tag不影响RSV重组蛋白G1F/M2的免疫原性
- Author:
Ruihong ZENG
;
Weihua WANG
;
Guizhen FANG
;
Wei GONG
;
Xingguo MEI
;
Lin WEI
- Publication Type:Journal Article
- Keywords:
Respiratory syncytial virus;
His-tag;
Recombinant protein G1F/M2;
Immunogenicity
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether His-tag to change the immunogenicity of recombinant protein G1F/M2 of respiratory syncytial virus.Methods:The G1 and F/M2 gene fragments were amplified by PCR method and then ligated into the expressing vector pET-His or pET-DsbA-His.Each recombinant plasmid was transferred into E.coli BL21(DE3) and the expression was induced by IPTG.The expressed His-G1F/M2 or DsbA-His-G1F/M2 was purified by affinity chromatography.The latter was digested with thrombase and G1F/M2 was purified by affinity chromatography.His-G1F/M2 or G1F/M2 was used to immunize BALB/c mice.Anti-RSV antibody was measured by ELISA and RSV-specific CTL responses by MTT.Results:No significant difference was observed between the level of anti-RSV antibody or RSV-specific CTL response induced by G1F/M2 and that by His-G1F/M2.Conclusion:His-tag does not change the immunogenicity of recombinant protein G1F/M2 of respiratory syncytial virus.
