Effects of invigorating the kidney,promoting qi and activating blood method in repairing articular cartilage defects in rabbits with induced chondrocytes by bone marrow matrix stem cell
- VernacularTitle:补肾行气活血法在骨髓基质干细胞诱导软骨细胞修复兔关节软骨缺损中的作用
- Author:
Xiaohui ZHENG
;
Yonggang TU
;
Xiongzhi XIA
- Publication Type:Journal Article
- Keywords:
qi;
chondrocyte;
cartilage defect;
bioengineering;
transplant
- From:
Orthopedic Journal of China
2006;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore the effects of invigorating the kidney,promoting qi and activating blood method in repairing articular cartilage defects in rabbits with chodrocytes induced by bone marrow matrix stem cell.[Method]The complexes from seeding of chondrocytes and allograft chondrocytes encapsulated in collagen gel and cultured in Guyanding drug serum into CPPf/PLLA scaffolds were cultured in vitro for 3 weeks. Some complexes were observed with the phase-contrast microscope or scanning electron microscope and others were allotransplanted into articular cartilage defects in rabbits.The specimens harvested at 4,8,12 weeks were analyzed by observation,histology and type Ⅱ collagen immunohistochemistry.[Result]The induced chondrocytes were embedded in scaffolds evenly the formation,smoothness of the surface and integration with adjacent tissues of the hyaline cartilage-like tissues,distribution of type Ⅱ collagen in the matrix of the induced chondrocytes group were better than all those in the chondrocyte group.[Conclusion]The method of invigorating the kidney,promoting qi and activating blood has superiority in repairing articular cartilage defects over the traditional method.
