Experiment study on compound ink Dabble-Van Gieson dyeing applied to osseous tissue capillary vessel imaging
- VernacularTitle:不同方法显像骨组织微血管的对比研究
- Author:
Yuekui JIAN
;
Xuehui WU
;
Xiaobin TIAN
- Publication Type:Journal Article
- Keywords:
capillary vessel;
ink;
Van Gieson dying;
compound
- From:
Orthopedic Journal of China
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To find a simple and direct feasible method to detect bone tissue vascularize from comparing advantage or disadvantage of ink intravascular Dabble-VG dyeing and other tissue dyeing in the tissue section capillary vessel display.[Methods]One side femoral artery of 6 normal goat was mixed with liquor composed of 10 percentink,10 percent formaldehyde and 20 percent mannitol,volume ratio in 7∶2∶1.Stopped femoral vein effluent was black.To get off goat tibia,10 percent formaldehyde was fixed with.EDTA decalcification,cedar oil douse,fiat 50 to 100 micrometer pachy-section and compound ink-VG dyeing to observe capillary vessel.[Results]Compound ink-VG dyeing ould display relations of capillary vessel and surrounding tissue.But in control group,single HE,Masson and Weigert Resorcinol-aniline red dyeing after-stain VG dyeing ould display few capillary vessel and surrounding tissue,and recognize difficultly.[Conclusion]Compound ink-VG dyeing can display capillary vessel of bone clearly,and can disclose relationships of surrounding tissue as well.This method can try on in tissue vascularization detect.
