Effect of Liuwei Dihuang decoction and its decomposed recipes on gene expression related to thymocytes differentiation in normal mice and SAMP8
- VernacularTitle:六味地黄汤及其拆方对正常小鼠和SAMP8胸腺淋巴细胞分化相关基因表达的影响
- Author:
Minggang BI
;
Wenxia ZHOU
;
Chunhui QI
;
Yongxiang ZHANG
- Publication Type:Journal Article
- Keywords:
Liuwei Dihuang decoction;
Immunosenescence;
Senescence-accelerated mouse;
Thymic lymphocytic subsets;
Real-time quantitative reverse transcriptase polymerase chain reaction;
Notch signal transduction pathway
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To research the effect of Liuwei Dihuang decoction and its decomposed recipes on gene expression related to thymocyte differentiation in normal mice and SAMP8.Methods:The expression of Notch1,Presenilin1,Presenilin2,HES1 mRNA was detected by quantitative real-time fluorescence PCR assay.Results:After oral administation of Liuwei Dihuang decoction(5,10,20 g/kg),the expressions of Presenilin2 and HES1 gene in thymocytes of normal mice were increased.After oral administation of Liuwei Dihuang decoction(10 g/kg)and SB,SX,the expressions of Presenilin2 and HES1 in thymocytes of normal mice were decreased.Conclusion:It is suggested that Liuwei Dihuang Decoction can increase the Notch signal intensity in the normal mice thymocytes and decrease that in SAMP8,which is immuno-senescenced mice.
